![]() The concentration of cGMP was determined by Radioimmunoassay (RIA). Water content of myocardial tissue was assessed by dry-wet weight, and cardiomyocytes water permeability was also calculated. Left ventricular function of each group was determined by pressure-volume system. Methods: ODQ, a specific inhibitor of soluble guanylate cyclase (sGC), was used to treat the CPB animals or cardiomyocytes. ![]() In our study, we used sheep CPB model of in vivo and in vitro to assess the relationship between cGMP and AQP1 during CPB. ![]() CPB can cause profound alterations V in the homeostasis of physiological fluids, which often results in myocardial edema. Background/Aims: Most cardiac procedures involve the use of cardiopulmonary bypass (CPB), which pumps oxygenated blood to the body while the heart and lungs are isolated.
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